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ATCC
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Image Search Results
Journal: Cell Death & Disease
Article Title: Zebularine regulates early stages of mESC differentiation: effect on cardiac commitment
doi: 10.1038/cddis.2013.88
Figure Lengend Snippet: Characterization of HS-181 cell line after treatment with zebularine. ( a ) RT-PCR of cardiac markers after treatment with zebularine. Myh7, Myh6, Actc, cTnI and Serca2 present more expression after treatment. ( b ) Immunostaining of treated cells to detect cardiac-specific proteins. Scale bars; 50 μ m. ( c ) Blots of Proteome Profiler Array and the resulting quantification histograms demonstrating inhibition of pluripotency marker expression and ( d ) increased levels of mesodermic proteins after zebularine treatment (black arrows)
Article Snippet: Protein expression profiles were assayed using the specific human pluripotent Stem Cell array kit
Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Immunostaining, Inhibition, Marker
Journal: BMC Cancer
Article Title: Tyrosine kinase inhibitor SU11274 increased tumorigenicity and enriched for melanoma-initiating cells by bioenergetic modulation
doi: 10.1186/s12885-016-2341-y
Figure Lengend Snippet: Bioenergetic modulation counteracts the protumorigenic action of the SU11274. a – b Protein lysates were prepared from untreated controls and Rel3 cells treated with 1 μM SU11274 in spheroid conditions for 7 days. Pluripotent stem cell array has shown expression of all pluripotency markers in Rel3 cells expanded as spheroids. SU11274 treatment further increased level of proteins associated with pluripotency in SU11274-treated cells correlating with their higher tumor initiating properties. Phosphokinase assay screening has shown several active intracellular signaling cascades. Phosphorylated forms of p53 (S392 and S46) were detected in the SU11274-treated cells (lower panel B), which correlates with SU11274 mediated inhibition of cell proliferation. RSK1/2/3 (S380) phosphorylation was increased in SU11274-treated cells. We detected phosphorylation of the following target kinases: ERK1/2 (T202/Y204, T185/Y187), P-RAS40 (T246), Akt 1/2/3 (S473), p38 alpha (T180/Y182), AMPK alpha1 (T183), CREB (S133), GSK-3 alpha/beta(S21/S9), WNK-1 (T60) and HSP60 in both treated and untreated cells
Article Snippet: Proteome profile of the melanosphere cells cultured for 7 days in the presence of 1 μM SU11274 was evaluated by the Proteome ProfilerTM Human Phospho-Kinase Antibody Array and the
Techniques: Expressing, Inhibition, Phospho-proteomics